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Summary Attributions Wiki
XB-GRNA-23659134
gRNA Name: amer3 gRNA1
Synonyms:
Genomic Target: amer3

PAM Sequence: GGG
Sequence: 5' GTCAGGGACATTTCAAGGTG 3'



Genomic Target tropicalis laevis.L
Identities 20/20 19/20
genome
gRNA
 
5' CACCTTGAAATGTCCCTGAC 3'
   ||||||||||||||||||||
3' GTGGAACTTTACAGGGACTG 5'
 
5' CACCTTGAAATGTCCCAGAC 3'
   |||||||||||||||| |||
3' GTGGAACTTTACAGGGACTG 5'
     
Genomic Alignments Position Identity Strand Genomic Target
laevis 10.1 Chr5L:136522920..136522939 19/20 Sense target amer3.L
Chr3L:14860359..14860378 17/20 Antisense off-target iqsec3.L
Chr6S:100394960..100394979 17/20 Antisense off-target atp6v1h.S
Chr1L:91113415..91113434 16/20 Antisense off-target ptpn13.L
Chr9_10S:112315926..112315945 16/20 Sense off-target LOC121399004
Chr5S:114497675..114497694 19/20 unknown
Chr6S:30956553..30956572 18/20 unknown
Chr8S:21857679..21857698 18/20 unknown
Chr9_10L:8132093..8132112 18/20 unknown
Chr6S:69355518..69355537 17/20 unknown
Chr1S:78231139..78231158 16/20 unknown
Chr5L:104525365..104525384 16/20 unknown
Chr9_10S:112593418..112593437 16/20 unknown
tropicalis 10.0 Chr5:126986653..126986672 20/20 Sense amer3
Chr2:154076248..154076267 18/20 Sense medag
Chr2:154076248..154076267 18/20 Antisense LOC105946588
Chr9:52586818..52586837 16/20 Antisense nbeal1
Chr6:26223466..26223485 18/20
Chr6:93718957..93718976 17/20
Chr2:99879091..99879110 16/20
Chr7:41878363..41878382 16/20
Publications
???morpholino.displaySummary.papersFirst??? Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos., Sci Rep 2020                        
???morpholino.displaySummary.papersRecent??? Identification and validation of candidate risk genes in endocytic vesicular trafficking associated with esophageal atresia and tracheoesophageal fistulas., HGG Adv 2022          
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