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Search Transgenic Constructs

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Transgene Name Description
Tg(dnmcidas)

A dominant negative form of Xenopus mcidas with the coiled-coil domain around amino acids 180-213 de...
Tg(dne2f4)

A form of Xenopus E2f4 with a myc epitope tag and nuclear localization signal (NLS) but lacking 140 ...
Tg(e2f4-GFP)

Fusion construct of Xenopus e2f4 and eGFP.
Tg(ctnnb1-FLAG)

A construct expressing xenopus ctnnb1 with a FLAG tag.
Tg(foxn4-GFP-GR)

A fusion protein of Xenopus foxn4 with the ligand binding domain of the human glucocorticoid recepto...
Tg(foxj1-FLAG)

This is a FLAG tagged form of Xenopus foxj1.
Tg(myb-GFP)

This is a fusion protein of Xenopus myb and GFP.
Tg(caHsa.bmpr1a)

A constitutively active form of human bmpr1a with a substitution of aspartic acid for glycine at am...
Tg(Flag-Mmu.map2k7-Hsa.mapk8)

This fusion protein encodes an NH2-terminal epitope tag (Flag), residues 1 to 443 of map2k7 (deletio...
Tg(GFP-rfx2)

A fusion protein consisting of GFP and Xenopus rfx2.
Tg(ctnnb1tm)

This encodes a phosphorylation defective form of Xenopus laevis ctnnb1. The specific mutations were...
Tg(dnbmp7.1)

Dominant negative form of bmp7.1 ligand with cleavage mutation blocking maturation. Conserved cleava...
Tg(dnsmad2)

The dominant-negative XSmad2 (P445H) was constructed by PCR mediated mutagenesis. This is a mutation...
Tg(pias4del)

This is a pias4 deletion construct with the RING domain between aa304 and 366 deleted. Also known as...
Tg(cagdf1)

To make Tg(CAgdf1), a DNA fragment encoding the first 288 amino acids of bmp2 was fused to a DNA fra...
Tg(HA-sox17bdel_168_1119)Zorn

This is an HA tagged form of Xenopus laevis sox17b with amino acids 56-373 deleted
Tg(HA-sox17bdel_1_405)Zorn

This is an HA tagged form of Xenopus laevis sox17b with amino acids 1-135 deleted.
Tg(HA-sox17bdel_1_597)Zorn

This is an HA tagged form of Xenopus laevis sox17b with amino acids 1-199 deleted. Also called d200-...
Tg(HA-sox17bdel_1_747)Zorn

This is an HA tagged form of Xenopus laevis sox17b with amino acids 1-249 deleted. Also called d250-...
Tg(Hsa.gmnc)

Tg(eef1a1.L:H2B-GFP)

The H2B-GFP (gift from Geoff Wahl, Addgene plasmid # 11680) was cloned into the downstream of the eu...
Tg(Gga.mylk-eGFP-Rno.calm)

GCaMP3 is a genetically encoded calcium indicator (GECI) created from a fusion of an artificial poly...
Tg(pitx3-IRES-GFP)

The pitx3 coding sequence was PCR-amplified from pBSK-pitx3 (NM_001088554) with primers harboring ad...
Tg(Mmu.th-DsRed)

pCS2-HcRED1 vector was generated through PCR of the HcRED1 sequence from pCAG-HcRED1 (Addgene collec...
Tg(snai1-GR)

Inducible DNA construct prepared by fusing the entire coding regions of Xenopus laevis snai1 (amino ...
Tg(six6:GFP)C1

Tg(pax3-GR)

pCS2 + pax3GR is a hormone inducible [via dexamethasone] construct in which Xenopus pax3 open readi...
Tg(zic1-GR)

Tg(zic1GR) is a hormone inducible [via dexamethasone ] constructs in which zic1 open reading frame i...
Tg(ctdnep1_D67E)

Phosphatase-inactive mutant form of ctdnep1.
Tg(ctdnep1_D69E)

Phosphatase-inactive mutant form of ctdnep1.
Tg(GR-mitf)

A hormone inducible form of mitf consiting of the human glucocorticoid ligand binding domain (GR) se...
Tg(dnkcnj2-dsRed)

A dominant negative form of kcnj2 mRNA with the deletion D71V mutation causes loss-of-function pheno...
Tg(Hsa.CLCN4m)

Human missense mutations in CLCN4 (p.Val212Gly, p.Leu221Pro, p.Val275Met, p.Ser543Leu, p.Ala555Val ...
Tg(Xtr.hal2:eGFP,cryga:GFP3)

X. tropicalis HAL2 promoter region was PCR amplified- the product was used for the nested PCR with p...
Tg(actc1:GFP)

An alpha--actin-driven construct (pα-actin::gal4-UAS::GFP), used to express GFP in neurons, after e...
Tg(sox2:eGFP)

named in 'pSox2bd::gal4-UAS::eGFP', the construct contains the Sox2/Oct3/4 enhancer elements of the ...
Tg(gatd3a-like:GFP)

A gatd3a-like GFP construct from the Mochii Lab
Tg(Hsa.SMCHD1R552Q)

Full length human SMCHD1 bearing a R552Q substitution associated with BOSMA ARHINIA MICROPHTHALMIA S...
Tg(Hsa.SMCHD1G137E)

Full length human SMCHD1 bearing a G137E substitution associated with BOSMA ARHINIA MICROPHTHALMIA S...
Tg(Hsa.SMCHD1W342S)

Full length human SMCHD1 bearing a W342S substitution associated with BOSMA ARHINIA MICROPHTHALMIA S...
Tg(Hsa.SMCHD1N524S)

Full length human SMCHD1 bearing a N524S substitution associated with BOSMA ARHINIA MICROPHTHALMIA S...
Tg(Hsa.SMCHD1H348R)

Full length human SMCHD1 bearing a H348R substitution associated with BOSMA ARHINIA MICROPHTHALMIA S...
Tg(Hsa.SMCHD1D420V)

Full length human SMCHD1 bearing a D420V substitution associated with BOSMA ARHINIA MICROPHTHALMIA S...
Tg(myod1-Mmu.GR-FLAG)

This construct contains the coding sequence of myod1 fused to the mouse glucocorticoid receptor bind...
Tg(sox5-VP16-GR-FLAG)

This construct contains the coding sequence of sox5 fused to the mouse glucocorticoid receptor bindi...
Tg(meox2-Mmu.GR-FLAG)

This construct contains the coding sequence of meox2 fused to the mouse glucocorticoid receptor bind...
Tg(meox2-GA-FLAG)

This construct contains the coding sequence of meox2 fused to a mutated form of the mouse glucocorti...
Tg(sox6-VP16-FLAG)

This construct contains the coding sequence of sox6 fused to the VP16 transactivation domain and a F...
Tg(sox6-EnR-FLAG)

This construct contains the coding sequence of sox6 fused to the drosophila engrailed repressor doma...
Tg(myod1-EnR-FLAG)

This construct contains the coding sequence of myod1 fused to the drosophila engrailed repressor dom...

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