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Fig. 2. Phosphorylation of the cytosolic regions of GIRK4 by PKA in-vitro.A.: upper panel: Autoradiogram showing 32P incorporation of the GIRK4 C- and N-terminal fusion proteins following incubation with PKA-cs and ATP-γ-32P. Lower panel: Coomassie stain of the electrophoresis gel shown in upper panel.B: Statistical analysis of 32P incorporation into N- and C-terminus. The number of individual phosphorylation experiments is shown in parenthesis above each bar. ***: the mean value deviates statistically significant from GST at the p < 0.001 level.

Image published in: Treiber F et al. (2013)

© 2013 Elsevier B. Creative Commons Attribution-NonCommercial-NoDerivatives license

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