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adam28.2xenopus head 

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Experiment details for adam28.2

Agricola ZN et al. (2015) Assay

Identification of genes expressed in the migrating primitive myeloid lineage of Xenopus laevis.

Gene Clone Species Stages Anatomy
adam28.2.S image:5513111 laevis NF stage 21 head

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  Figure 4. Animal cap-injected X.t. cebpa mRNA induces exogenous myeloid gene expression in Stage 21 X.l. embryos. A–J: Uninjected embryos ventral view. A′–J′: 200 pg cebpa and 300 pg gfp mRNA coinjected embryos ventral view. A, A′: spib.a. B, B′: mpo. C, C′: mmp7. D, D′: dstn. E, E′: coro1a. F, F′: ptpn6. G, G′: timp–1. H, H′: fmnl1. I, I′: ctss. J, J′: adam28.

Gene Clone Species Stages Anatomy
adam28.2.S image:5513111 laevis NF stage 23 head

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  Figure 3. Knockdown of primitive myeloid lineage spib.a transcription factor results in loss of myeloid gene candidate expression in the anterior ventral blood island (aVBI) of stage 23 embryos. A–J: Normal expression of genes in the aVBI of uninjected control embryos. A′–J′: Reduced aVBI expression of genes in the embryos injected dorsally at 4-cell stage with 40 ng e1i1 spib.a morpholino. A+A′, spib.a. B+B′, mpo. C+C′, mmp7. D+D′, adam28. E+E′, coro1a. F+F′, ctss. G+G′, dstn. H+H′, fmnl1. I+I′, timp-1. J+J′, ptpn6.

Gene Clone Species Stages Anatomy
adam28.2.S image:5513111 laevis NF stage 24 head
adam28.2.S image:5513111 laevis NF stage 28 head

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  Figure 1. Stage 15, 19, 24, and 28 expression of identified genes progress from anterior ventral blood island to dispersed distribution. They are temporally downstream of transcriptional regulators spib.a (A–A‴) but coincident with other, later expressing myeloid functional genes like mmp7 (B–B‴). C–C‴, adam28. D–D‴, coro1a. E–E‴, ctss. F–F‴, dstn. G–G‴, fmnl1. H–H‴, ptpn6. I–I‴, timp-1. White scale bar = 1 mm.

Gene Clone Species Stages Anatomy
adam28.2.S image:5513111 laevis NF stage 24 head

  adam28 (ADAM metallopeptidase domain 28) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 24, ventral view, anterior left