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Fig. 2. Xotx1 does not induce ectopic cement glands and, in coinjection
experiments, inhibits Xotx2 cement-gland-inducing ability.
Embryos at 2-cell stage were injected with 0.8 ng of DXotx1 (A),
Xotx1 (B) or Xotx2 RNA (C), or co-injected with 0.8 ng of Xotx1 and
0.8 ng of Xotx2 RNA (D) and the resulting stage 32 embryos were
subjected to whole-mount in situ hybridization with XAG-1, a probe
specific for the cement gland. Both DXotx1 (A) and Xotx1 (B)-
injected embryos display wild-type pattern of XAG-1 expression.
Embryos injected with Xotx2 RNA show ectopic expression of XAG-
1 (C, arrows) which is strongly repressed (D, leftmost embryo;
arrowhead point to residual ectopic XAG-1 expression) or completely
abolished (D, rightmost embryo) in embryos co-injected with both
Xotx1 and Xotx2. |
