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Fig. 3. Results of embryos microinjections and ag1 in situ hybridization. (A and B) A total amount of 800 pg of capped mRNAs corresponding to the different constructs as shown was ventrally (A) or dorsally (B) bilaterally microinjected in Xenopus embryos at 4-cell stage; embryos were grown to tailbud stage and processed by whole mount in situ hybridization with an ag1 probe. When microinjected dorsally (B), otx1, otx2 and otx5 induce posterior defects in Xenopus embryos; this activity is maintained by all constructs, but Xotx5-87δC. LacZ was coinjected as a tracer. Microinjection of LacZ alone was used as negative control. |
