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Figure 9. FoxA4 depletion altered neurogenesis along the A–P axis.
(A–J) N-tubulin and Xag1 expression in stage 27/28 embryos injected with 40 ng of CtrlMO (A, C, E–G) or FoxA4MO (B, D, H–J) into one cell at the 2-cell stage. (A, B) Dorsal views. (C, D) Magnified images of the trunk region of another pair of CtrlMO (C) and FoxA4MO (D) injected embryos, shown in dorso-lateral views to facilitate comparisons. The red arrow points to an N-tubulin + projection on the injected side. (E, F, H, I) Lateral views of the head region. (G, J) Anterior views. N-tubulin is expressed in the trigeminal (tn) and vestibulocochlear (vn) nerves. In FoxA4MO-injected embryos, the tn and vn were disturbed (red arrowhead) (H), as revealed by N-tubulin; the cement gland (cg) was bent and closer to the forebrain, and the hatching gland (hg) was shortened on the FoxA4-depleted side, as revealed by Xag1 (green arrowhead) (J) (90%, n = 21). (K–N) Transverse sections at the levels indicated by dotted black lines in C,D, shown in bright field (K, M) and their corresponding nuclear Hoescht fluorescence (L, N). Bright field images were processed with Adobe Photoshop CS2 in order to superimpose the N-tubulin expression (yellow) to the Hoescht fluorescence. Yellow arrows point to the projection emerging from the dorsal neural tube; cyan arrows point to both sides of the ventral neural tube; green arrows point to the place left by the disorganised or absent notochord; no, notochord; is, injected side; nis, non-injected side. (O, P) Summary of the results shown in A–J.
doi:10.1371/journal.pone.0110559.g009 |
