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Figure 3. Twisted-Gastrulation Is Required for the Effects of CV2 Loss of Function and Overexpression(A) Simultaneous depletion of CV2 and Chordin strikingly increased CV2 expression, reflecting increased BMP signaling (see Figure S5 for controls).(B) The effects of CV2 MO and Chd MO require Twisted-Gastrulation (Tsg) activity (pro-BMP effect of Tsg).(C) CV2 protein injection into the blastula cavity induces strong dorsalization of the Xenopus embryo, as indicated by the expansion of the dorsal markers Xag1, Six3, and Krox20 (n = 12, all strongly dorsalized). Inset shows an uninjected embryo.(D) CV2 protein requires endogenous Tsg for its anti-BMP activity (n = 10, all embryos similarly affected). Inset shows Tsg MO-injected embryo.(E) CV2 protein injection expands the neural tube (n = 17). Inset shows uninjected embryo.(F) Tsg and CV2 protein coinjection renders CV2 a stronger BMP antagonist, expanding the nervous system marked by Sox3 (n = 16, all coinjected embryos were more dorsalized than those injected with CV2 protein alone despite some individual variations). Inset shows embryo injected with Tsg protein alone. |
