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Fig. 4. Expression of BmprII mRNA in the developing Xenopus eye. (A) Stage 30 Xenopus embryo processed for BmprII in situ hybridization. BmprII transcripts are expressed in the eye (outlined by dotted line). (B,C, E,F) Ubiquitous expression of BmprII mRNA in the stage 28 retina (B) becomes refined to the inner layers of the neural retina and the lens at stage 30 (C), 33/34 (E) and 35/36 (F). Expression is absent from the emerging photoreceptors in the outer nuclear layer (onl). (D) A retina immunolabeled for Islet-1 shows the location of the RGCs in the inner- most layer of a stage 33/34 retina. Scale bar in (B) is 50 μm for panels (B- F). Abbreviations: D, dorsal; V, ventral; h, heart; L, lens; nr, neural retina; onl, outer nuclear layer; ot, otic vesicle; rgcl, retinal ganglion cell layer; rpe, retinal pigment epithelium. |
