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cdk9xenopus   

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Experiment details for cdk9

The positive transcriptional elongation factor (P-TEFb) is required for neural crest specification.

The positive transcriptional elongation factor (P-TEFb) is required for neural crest specification.

Gene Clone Species Stages Anatomy
cdk9.S laevis NF stage 16 neural crest , neural plate , neural plate border
cdk9.L laevis NF stage 16 neural crest , neural plate , neural plate border
cdk9.S laevis NF stage 18 neural crest , neural plate , neural plate border
cdk9.L laevis NF stage 18 neural crest , neural plate , neural plate border
cdk9.S laevis NF stage 26 brain , pharyngeal arch , head
cdk9.L laevis NF stage 26 brain , pharyngeal arch , head
cdk9.L laevis NF stage 28 brain , branchial arch , head
cdk9.S laevis NF stage 32 brain , branchial arch , head

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  Fig. 1. The expression of the P-TEFb complex components during Xenopus laevis development. Transcripts for the P-TEFb components were detected using NBT/BCIP and expression of CDK9.S, CDK9.L and CyclinT1 appeared blue in the sectioned neurula stage embryo. Sox10 was detected using fast red and fluorescence microscopy. (A) CDK9.S expression at stage 16 and 18 in the neural plate and neural plate border and expression at stages 26 and 32 in the branchial arches. Scale bar=100 µm (Bi) 10× magnification section of a stage 16 embryo showing CDK9.S expression in the neural plate and neural plate border (blue) and Sox10 expression in the neural crest (red). (ii) 20× magnification section shows overlap of the blue CDK9.S expression with the red Sox10 expression. Expression of CDK9.S is outlined with a dashed white line and overlaid onto the Sox10 expression image. Scale bar=100 µm (C) CDK9.L expression at stage 16 and 18 in the neural plate and neural plate border and expression at stages 26 and 32 in the branchial arches. Scale bar=100 µm (Di) 10× magnification section of a stage 16 embryo showing CDK9.L expression in the neural plate and neural plate border (ii) 20× magnification section shows overlap of the blue CDK9.L expression with the red Sox10 expression. Expression of CDK9.L is outlined with a dashed white line and overlaid onto the Sox10 expression image. Scale bar=100 µm (E) CyclinT1 expression at stage 15 and 18 in the neural plate and neural plate border and expression at stages 26 and 32 in the branchial arches. Scale bar=100 µm (Fi) 10× magnification section of a stage 15 embryo showing CDK9.S expression in the neural plate and neural plate border (blue) and Sox10 expression in the neural crest (red). (ii) 20× magnification section shows overlap of the blue CDK9.S expression with the red Sox10 expression. Expression of CDK9.S is outlined with a dashed white line and overlaid onto the Sox10 expression image. Scale bar=100 µm. Npb=Neural plate border, ba=branchial arches.