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Figure 2. RARα and CBP [crebbp] expression in presumptive neural crest in Xenopus Laevis. (A) Developmental expression of the RARα and CBP proteins in Xenopus laevis. β-actin was used as an internal control. Statistics revealed the ratio of protein levels relative to stage 2. RARα (B) and CBP (G) are maternally expressed at the animal hemisphere, and the expression intensity increases at stage 7 (C,H). At the neural plate stage (stage 15), RARα (D) and CBP (I) appear in the central neural plate (* white asterisk means neural plate) and anterior neural plate border (black arrowhead). At stage 25 (E) and stage 30 (F), RARα is in the neural tube and branchial arch (red arrowhead). CBP has a similar expression pattern in the branchial arch (red arrowhead) and neural tube at stage 25 (J) and stage 30 (K). np, neural plate; b, neural plate border region; ba, branchial arch. Scale bar: 100 μm. |