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cyp26c1xenopus anterior placodal area [+] 

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Experiment details for cyp26c1

Tanibe M et al. (2008) Assay

Retinoic acid metabolizing factor xCyp26c is specifically expressed in neuroectoderm and regulates anterior neural patterning in Xenopus laevis.

Gene Clone Species Stages Anatomy
cyp26c1.S laevis NF stage 13 anterior placodal area

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  Fig. 5. Effect of retinoic acid (RA) on xCyp26c expression. (A-X) Embryos were treated with various doses of RA at stage 8, cultured until stage 13, and then prepared for WISH analysis. No treatment (A-C). RA treatment (D-X). Concentration of RA (M) is shown on left side. Expres- sion pattern of xCyp26c (A-E), xCyp26a (I-P) and xOtx2 and xkrox20 (Q- X). Moderate doses of RA increased xCyp26c expression, but narrowed the area of expression (A-M). Over 10-6 M of RA, xCyp26c expression became restricted in the anterior neuroectoderm and underwent no further change (F-H). XCyp26a was expressed throughout the embryo (O,P), whereas xOtx2 expression completely disappeared (V-X). (Y) Real-time PCR analysis with whole embryos treated with RA. These graphs are expressed as ratio of expression level of RA-treated embryo (lane 2-7) to that of no-RA treated embryo (lane 1).