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cyp26c1xenopus cement gland primordium [+] 

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Experiment details for cyp26c1

Tanibe M et al. (2008) Assay

Retinoic acid metabolizing factor xCyp26c is specifically expressed in neuroectoderm and regulates anterior neural patterning in Xenopus laevis.

Gene Clone Species Stages Anatomy
cyp26c1.S laevis NF stage 13 cement gland primordium

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  Fig. 5. Effect of retinoic acid (RA) on xCyp26c expression. (A-X) Embryos were treated with various doses of RA at stage 8, cultured until stage 13, and then prepared for WISH analysis. No treatment (A-C). RA treatment (D-X). Concentration of RA (M) is shown on left side. Expres- sion pattern of xCyp26c (A-E), xCyp26a (I-P) and xOtx2 and xkrox20 (Q- X). Moderate doses of RA increased xCyp26c expression, but narrowed the area of expression (A-M). Over 10-6 M of RA, xCyp26c expression became restricted in the anterior neuroectoderm and underwent no further change (F-H). XCyp26a was expressed throughout the embryo (O,P), whereas xOtx2 expression completely disappeared (V-X). (Y) Real-time PCR analysis with whole embryos treated with RA. These graphs are expressed as ratio of expression level of RA-treated embryo (lane 2-7) to that of no-RA treated embryo (lane 1).

Gene Clone Species Stages Anatomy
cyp26c1.S laevis NF stage 24 cement gland primordium
cyp26c1.S laevis NF stage 28 cement gland primordium

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  Fig. 3. Spatial expression of xCyp26c after tailbud stage. (A-B, D-E, G-H) XCyp26c expression. (C,F,I) XCyp26a expresion. Stage 24 (A-C), stage 28 (D-F), and stage 36 (G- I). XCyp26c was expressed in restricted sets of cranial nerves (GC(VII) and GC(IX)). XCyp26c was also expressed in the pharyngeal pouch (PP) and rhombomeric cells (r2/3 and r6). These patterns were obviously different from those of xCyp26a (C,F,I).

Gene Clone Species Stages Anatomy
cyp26c1.S laevis NF stage 24 cement gland primordium

  cyp26c1 ( cytochrome P450, family 26, subfamily C, polypeptide 1 ) gene expression in Xenopus laevis embryo, NF stage 24, as assayed by in situ hybridization, lateral view, anterior left, dorsal up.