||Fig. 1. Expression of x-Dll3, x-Nkx2.1, and x-Lhx7 in embryonic Xenopus forebrain. The x-Nkx2.1 protein and GABA neurotransmitter are visualized by
immunofluorescence (IF), and the x-Lhx7 and x-Dll3 transcripts are detected by in situ hybridization (ISH, red) revealed by Fast Red and observed in fluorescence
(x-Lhx7) or in bright field microscopy (x-Dll3). Panels A–C show lateral views of in toto dissected brains stained with the indicated gene marker at stage 35.
Anterior is left, dorsal is up, and the shape of the brain is outlined by a dotted line for fluorescence pictures. (D) Optical and confocal sections through the basal
telencephalon of stage 35 embryos labeled for x-Dll3 mRNA or double-labeled for x-Nkx2.1 protein and x-Lhx7 mRNA. Dotted lines delineate subdivisions and
neuroepithelial layers. Hyp, hypothalamus; mge, lge, medial and lateral ganglionic eminence; pa, pallium; spa, subpallium; vz/svz/mz, ventricular, subventricular,
and marginal zone.