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Fig. 1. Colocalization of the DM-W and DMRT1 proteins in somatic cells surrounding the PGCs of the ZW gonad at stage 50. (A) In situ hybridization analyses of DM-W, DMRT1 and XVLG-1 in cross-sections of primordial ZW (upper) or ZZ (lower) gonads at stage 50. Asterisks indicate primordial germ cells (PGCs). Scale bars: 5 μm. (B) Western blot analysis using the anti-DM-W and anti-DMRT1 antibodies. 293T cells were transiently transfected with pcDNA3-FLAG-DM-W or pcDNA3-FLAG-DMRT1. The extracts of the 293 cells (left) and primordial ZZ and ZW gonads (stages 50-52) (right) were examined by immunoblotting with each antibody followed by an HRP-conjugated anti-rabbit antibody. DM-W was only detected in the ZW samples. (C) Immunofluorescence analysis using anti-DM-W and anti-DMRT1 antibodies on cross-sections of primordial ZW gonads at stage 50. Scale bars: 2 μm. Asterisk indicates a PGC. |