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Fig. 2. Expression of DMRT1 during development in X. laevis.
(A) Reverse transcription-polymerase chain reaction (RT-PCR)
analysis of DMRT1 and elongation factor-1α (EF-1α) and
ornithine decarboxylase (ODC) as a control in unfertilized eggs
and embryos from stage (st.) 2-52. Each sample contained
0.25 μg of total RNA. L, H, P, M and G show liver, heart,
pronephros, mesonephros and primordial gonads, respectively.
(B) Specific expression of DMRT1 in the primordial gonad.
Whole-mount in situ hybridization using the antisense or sense
RNA probe for DMRT1 (nucleotides 598–1287) was performed
in tadpoles at stage 52 during sex determination. f and h show
forelimb and hindlimb. Scale bars, 0.5 mm. |
