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Fig. 2. Distribution of Dmrt1 in ZZ and ZW gonads during tadpole development. Immunostaining with anti-Dmrt1 and anti- VASA antibodies was performed on gonadal sections from ZW and ZZ tadpoles at stages 56 (A) and 62 (B). Nuclei were stained with TO-PRO-3. Oc, oocyte; Og, secondary oogonium; POg, primary oogonium; PSg, primary spermatogonium; pSe, pre-Sertoli cell. White arrowheads and arrows indicate Dmrt1-expressing pre- Sertoli cells and primary oogonia, respectively. Typical GSCs with somatic cells and their 2.59 magnification images are shown in the dashed squares. A dashed polygon indicates a typical cyst consisting of secondary oogonia. |
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Fig. 5. Distribution of cH2AX in ZZ and ZW gonads during development. Immunostaining with anti-Dmrt1 and anticH2AX antibodies was performed on gonadal sections from ZW and ZZ tadpoles at stages 53 and 62 (A) and on gonadal sections from a ZZ adult frog (B). Nuclei were stained with TO-PRO. Oc, oocyte; POg, primary oogonium; PSg, primary spermatogonium; Sg, secondary spermatogonium; Sc, spermatocyte. White arrowheads and arrows indicate Dmrt1- or cH2AX-positive primary GSCs and leptotene/zygotene oocytes, respectively. |
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Fig. 3. Distribution of Dmrt1 in the immature ovary and mature testis after metamorphosis. Immunostaining with anti- Dmrt1 and anti-VASA antibodies was performed using the immature ovary from a ZW frog 2 weeks after metamorphosis (A) and the mature testis of an adult ZZ frog (B). Nuclei were stained with TOPRO- 3. Oc, Oocyte; POg, primary oogonium; PSg, primary spermatogonium; Se, Sertoli cell; Sg, secondary spermatogonium; Sc, spermatocyte; St, spermatid. pSe, pre-Sertoli cell. Dmrt1- expressing primary oogonia and their 59 magnification images are shown in the dashed squares. White arrows and arrowhead in (A) indicate primary oogonia and diplotene oocytes, respectively. White arrowheads in (B) indicate Dmrt1- expressing primary spermatogonium. |
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Fig. 4. BrdU incorporation and Dmrt1 expression in the ZW developing ovary and ZZ adult testis. Immunostaining with anti-Dmrt1 and anti-BrdU antibodies was performed on the immature tadpole ovary at stage 62 and the mature testis of an adult frog. Nuclei were stained with TOPRO- 3. Og, secondary oogonium; POg, primary oogonium; PSg, primary spermatogonium; Sg, secondary spermatogonium; Sc, spermatocyte. |
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dmrt1 (doublesex and mab-3 related transcription factor 1) gene expression in Xenopus laevis testis, assayed via immunohistochemistry, adult frog stage. |
