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Fig. 4. BrdU incorporation and Dmrt1 expression in the ZW developing ovary and ZZ adult testis. Immunostaining with anti-Dmrt1 and anti-BrdU antibodies was performed on the immature tadpole ovary at stage 62 and the mature testis of an adult frog. Nuclei were stained with TOPRO- 3. Og, secondary oogonium; POg, primary oogonium; PSg, primary spermatogonium; Sg, secondary spermatogonium; Sc, spermatocyte. |
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Gene |
Clone |
Species |
Stages |
Anatomy |
dmrt1
|
|
laevis
|
NF stage 53
|
testis
,
ovary
,
primordial germ cell
|
dmrt1
|
|
laevis
|
NF stage 62
|
ovary
,
primary oogonium
,
secondary oogonium
,
primary spermatogonium
|
h2ax
|
|
laevis
|
NF stage 54
to
prometamorphosis stage
|
testis
,
ovary
,
primordial germ cell
|
h2ax
|
|
laevis
|
NF stage 62
|
testis
,
oocyte
,
ovary
|
h2ax
|
|
laevis
|
adult frog stage
|
testis
,
primary spermatogonium
,
spermatocyte
,
secondary spermatogonium
|
|
|
Fig. 5. Distribution of cH2AX in ZZ and ZW gonads during development. Immunostaining with anti-Dmrt1 and anticH2AX antibodies was performed on gonadal sections from ZW and ZZ tadpoles at stages 53 and 62 (A) and on gonadal sections from a ZZ adult frog (B). Nuclei were stained with TO-PRO. Oc, oocyte; POg, primary oogonium; PSg, primary spermatogonium; Sg, secondary spermatogonium; Sc, spermatocyte. White arrowheads and arrows indicate Dmrt1- or cH2AX-positive primary GSCs and leptotene/zygotene oocytes, respectively. |
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dmrt1 (doublesex and mab-3 related transcription factor 1) gene expression in Xenopus laevis testis, assayed via immunohistochemistry, adult frog stage. |