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dmrt1xenopus secondary spermatogonium [+] 

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Experiment details for dmrt1

Fujitani K et al. (2016) Assay

Sexually dimorphic expression of Dmrt1 and γH2AX in germ stem cells during gonadal development in Xenopus laevis.

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Gene Clone Species Stages Anatomy
dmrt1.L laevis adult frog stage secondary spermatogonium

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  Fig. 4. BrdU incorporation and Dmrt1 expression in the ZW developing ovary and ZZ adult testis. Immunostaining with anti-Dmrt1 and anti-BrdU antibodies was performed on the immature tadpole ovary at stage 62 and the mature testis of an adult frog. Nuclei were stained with TOPRO- 3. Og, secondary oogonium; POg, primary oogonium; PSg, primary spermatogonium; Sg, secondary spermatogonium; Sc, spermatocyte.

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Gene Clone Species Stages Anatomy
dmrt1.L laevis adult frog stage secondary spermatogonium

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  Fig. 5. Distribution of cH2AX in ZZ and ZW gonads during development. Immunostaining with anti-Dmrt1 and anticH2AX antibodies was performed on gonadal sections from ZW and ZZ tadpoles at stages 53 and 62 (A) and on gonadal sections from a ZZ adult frog (B). Nuclei were stained with TO-PRO. Oc, oocyte; POg, primary oogonium; PSg, primary spermatogonium; Sg, secondary spermatogonium; Sc, spermatocyte. White arrowheads and arrows indicate Dmrt1- or cH2AX-positive primary GSCs and leptotene/zygotene oocytes, respectively.

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Gene Clone Species Stages Anatomy
dmrt1.L laevis adult frog stage secondary spermatogonium

  dmrt1 (doublesex and mab-3 related transcription factor 1) gene expression in Xenopus laevis testis, assayed via immunohistochemistry, adult frog stage.