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Supplemental Figure 2: Characterization of Dsp expression. A) RT-PCR of Dsp at embryonic stages as well positive controls in the adult skin and heart. RT+ = reverse transcriptase was added, RT- = reverse transcriptase was not added. Primer sequences available upon request. B-E''') Immunofluorescence of Dsp (green) in the epidermis from various embryonic stages. Dapi (blue) and beta-catenin (pink) labeling were used as a counter stain. F-F''') A 3D rendering of images as shown in E-E''' were created and then rotated 90 degrees. G-G''') st. 30 where only the inner epidermal layer of cells are shown. All scale bars = 40µm |
