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Fig. 1. Isolation of X17C cDNA by differential display, and deduced amino acid sequence of X17C protein. (A) Display of amplified cDNAs on a denaturing polyacrylamide gel. The cDNA were derived by RT-PCR, using arbitrary primers (see Experimental procedures) from stage 13 embryos treated as follows: (I) control untreated; (2) retinoic acid treated; (3) retinoic acid plus cycloheximide treated. The arrow indicates the initially-isolated X17C cDNA fragment. The approximate positions of markers at 182 and 276 nucleotides are indicated. (B) Deduced amino acid sequence of the XI7C protein. (C) Comparison of X17C with the CLlOO MAP kinase phosphatase (Clustal V program). |