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Fig. 3. Posterior hox gene expression in regeneration requires Hif1α and Wnt. A) Pie chart of hox gene expression changes following 2ME, Ech, or IWR treatment. B) Schematic representation of hox gene organization in Xenopus. Color coding is as in (A). C) Schematic depicting hox gene expression in tailbud stage tadpoles. Color coding is as in (A). Boundaries were determined by lining up in situ expression patterns from Xenbase at the noted stages and using structural landmarks to determine the anterior and posterior domains. D) Heatmap displaying all detected hox genes across a regeneration timecourse. E) Heatmap displaying all detected hox genes clustered by expression. Genes significantly differentially expressed in all treatments are indicated by ∗. F) in situ hybridization for selected hox genes at 24hpa following treatment with DMSO, 2ME, Ech, or IWR. Arrowheads indicate amputation plane. Scale bars in E are 150 μm. |
