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hoxc9-likexenopus   

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Experiment details for hoxc9-like



BMP antagonists and FGF signaling contribute to different domains of the neural plate in Xenopus.

Gene Clone Species Stages Anatomy
hoxc9-like.L laevis NF stage 22 spinal cord , posterior neural tube

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  Fig. 6. Anterior neural markers are more sensitive to the loss of Bmp antagonists than posterior neural markers. X. laevis embryos were injected at the two-cell stage with increasing doses of Follisatin, Chordin, and Noggin MOs. Embryos were injected in both blastomeres at the two-cell stage; doses indicated refer to the total dose of MO injected into the embryo. Embryos were raised to stage 22, fixed and analyzed by in situ hybridization for expression of the genes indicated. nkx2.1 marks the ventral forebrain and is lost in embryos injected with low doses of FCN MOs. The forebrain/midbrain marker otx2, midbrain/hindbrain boundary marker en2 and rhombomeres 3/5 marker krox20 have reduced expression in embryos injected with low or intermediate doses of MO, and are lost in embryos injected with a high dose of MOs. The spinal cord marker hoxB9 is expressed strongly in embryos injected with low or intermediate doses of MO and is lost in embryos injected with a high dose of MO. Embryos are shown in lateral views with anterior to the left.