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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
hoxb3
|
|
laevis
|
NF stage 27
|
hindbrain
,
spinal cord
,
neural crest
,
pharyngeal arch
,
hyoid arch
,
[+]
|
hoxb1
|
|
laevis
|
NF stage 28
|
branchial arch
,
eye
,
hypobranchial placode
,
rhombomere R4
|
hoxb5
|
|
laevis
|
NF stage 31
to
NF stage 32
|
otic vesicle
,
midbrain
,
spinal cord
,
pharyngeal arch
,
hyoid arch
,
[+]
|
hoxb4
|
|
laevis
|
NF stage 31
to
NF stage 32
|
otic vesicle
,
spinal cord
,
somite
,
pronephric duct
,
pharyngeal arch
,
[+]
|
hoxb7
|
|
laevis
|
NF stage 31
to
NF stage 32
|
otic vesicle
,
diencephalon
,
hindbrain
,
spinal cord
,
somite
,
[+]
|
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|
FIG. 1. In situ hybridization ofstage 28 (L B) of stage 31/2 (C-F) tailbud stage embryos using digoxigenin-labeled probes. Hybridization was visualized using an alkaline phosphatase reaction with nitroblue tetrazolium as the substrate. All embryos are shown on their sides with anterior to the left. (A) Hoxb-1. Staining is in the hindbrain adjacent to the otic vesicle. (B) Hoxb-3. The probe hybridized to the CNS immediately posterior to the otic vesicle and to posterior branchial arches. (C) Hoxb-4. Transcripts were detected in the hindbrain, in the fourth branchial arch, in somites, and in pronephric ducts. (D) Hoxb-5. (E) Hoxb-7. In both cases, the posterior-most part of the hindbrain, the spinal cord, the pronephric duets, and the tail bud were stained. Weak staining in the somites was also seen in controls. (F) Hoxb-9. Staining was detected in the spinal cord. The tail bud may also be weakly stained.
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