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Fig. 2. Hox gene expression during somitogenesis is affected by the downregulation of X-Delta-2. Non-injected controls (Nic; A,C,E) and embryos injected in the left-hand side (LHS) with X-Delta-2 morpholino (MO) (B,D,F) were analysed for Hoxb4 (A,B), Hoxc6 (C,D), and Hoxb9 (E,F) expression at tailbud stage (stage 21). Embryos are shown from the dorsal side with anterior to the top. All three Hox genes were downregulated but whilst Hoxb4 (B: 97%, n=31) and Hoxb9 (F: 100%, n=22) expression was severely downregulated along the AP axis, the posterior expression of Hoxc6 (D: 91%, n=23) was not affected. The arrowhead indicates the injected side of the embryo. Dotted line indicates the midline. |