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Figure 3. Smad10 Is Required for Expression of Anterior and Posterior Neural Markers and for Formation of Melanocytes(A) Embryos were injected and analyzed by in situ hybridization as in Figure 2B.(B) Albino embryos (albino mother, pigmented father) were examined for the appearance of melanocytes (MC; dark cells visible in the C MO embryo).(C) Embryos were injected and analyzed at early neurula by in situ hybridization as in Figure 2B, except that 12.5 ng of morpholino was injected per cell.(D) Animal caps were explanted at stage 8.5, prior to organizer signaling, and Sox-2 expression was analyzed by RT-PCR.(E) Two-cell embryos were injected with morpholinos and, at the 32-cell stage, β-galactosidase mRNA was injected into the B1 blastomere. At stage 27, the embryos were stained with X-gal (blue). |
