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Fig. 2. Dazap2 silencing results in a loss of hoxb9 with concomitant posterior shift of anterior neural markers. Embryos co-injected with Dazap2 SP-MO and lacZ mRNA were analyzed at stage 18 (shown in dorsal views, with anterior at the top). (A, B) Sox2 expression domain was only marginally affected. (C, D) Otx2 domain was expanded and shifted posteriorly within the neural plate (blue and red lines indicate normal and shifted posterior limits respectively). (E, F) Krox20 expression (rhombomeres 3 and 5) also shows a posterior shift in SP-MO injected embryos (blue and red lines indicate normal and posteriorized rhombomeres 3 and 5 respectively). Expression of hoxb9 is decreased/lost in injected cells (G, H) and can be rescued when dazap2 mRNA (250 pg) is co-injected with SP-MO (I). The line indicates the sagittal midline |
