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Fig. 4. Cdx4 induction of hoxb9 does not require dazap2. Embryos were injected with either SP-MO (20 ng, B), cdx4 (250 pg, C), or a combination of SP-MO and cdx4 (20 ng and 250 pg, respectively, D) into one cell of 4-cell stages (all were co-injected with lacZ mRNA). Stage 18 embryos are shown in dorsal views, with anterior at the top. As previously stated, SP-MO blocks the endogenous expression of hoxb9 (B, arrow), but has no effect on cdx4-induced ectopic expression of hoxb9 (C and D, arrowheads). (E) Ectodermal explants show that cdx4 induces hoxb9 in Noggin-neuralized caps (lane 7) and this is not affected by the silencing of dazap2 (lane 8). WE: whole embryo, -RT: no reverse transcriptase, Ni: not injected |
