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Fig. 3. Phenotypes resulting from Coco overexpression in Xenopus embryos. Analysis of injected embryos at gastrula (A-D), neurula (H) and early tadpole stages (E-G,I-N). In A-D and H-N, the top panels are uninjected embryos, the lower panels are embryos injected with 1 ng Coco vegetally in 1 cell at the 2-cell stage (A-D) or ventrally in one cell at the 4-cell stage (G-N). (A) Xbra/brachyury (a marker of mesoderm); (B) Fgf8, also a mesoderm marker, (C) Otx2, (the organizer and anterior ectoderm); (D) gsc (organizer); (H) rx, (forebrain); (I) Emx1 (dorsal telencephalon); (J) Otx2, forebrain and midbrain; (K) En2 (midbrain/hindbrain boundary); (L) Hoxb9 (spinal cord); (M) Nkx2.5 (heart) (N) 12-101 Ab (muscle). (E-G) Phenotypes resulting from (E) no injection (control embryo); (F) dorsal animal injection at the 2-cell stage; (G) injection in one ventral vegetal cell at the 4-cell stage. *indicates the extra head structures. A,B,D are vegetal views; C, lateral with animal pole to the top; E-G,I-K, lateral views; H,L,N dorsal views; M, ventral view. In E-N, anterior is to the right. |
