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hoxd13xenopus   

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Experiment details for hoxd13

Regeneration-specific expression pattern of three posterior Hox genes.

Regeneration-specific expression pattern of three posterior Hox genes.

Gene Clone Species Stages Anatomy
hoxd13.S image:3399571 laevis NF stage 29 and 30 head region , mesoderm , presomitic mesoderm , tail bud , posterior neural tube , [+]
hoxd13.S image:3399571 laevis NF stage 51 to NF stage 55 mesoderm , anterior , limb bud

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  Figure 2. Posterior Hox gene expression in embryos and developing limbs. In situ hybridisation in stage 30 embryos and various stages of developing limbs. A-D: XHoxc10 expression. E-H: XHoxa13 expression. I-L: XHoxd13 expression. White arrows indicate anterior expression boundary in the CNS. For the limbs, the orientation is natural, i.e., the viewer is looking down dorsally, proximal is to the left, anterior is down, and posterior up.

Gene Clone Species Stages Anatomy
hoxd13.S image:3399571 laevis NF stage 29 and 30 head region , mesoderm , presomitic mesoderm , tail bud , posterior neural tube , [+]

  hoxd13 (homeobox D13) ggene expression in Xenopus laevis embryo via in situ hybridization, NF stage 29 & 30, lateral view, anterior left.

Gene Clone Species Stages Anatomy
hoxd13.S image:3399571 laevis NF stage 50 to NF stage 52 tail , neural tube , regenerating tail

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  Figure 4. Posterior Hox gene expression in regenerating and wounded tadpole tails. In situ hybridisation of posterior Hox genes after tail resection or wounding. A-C: XHoxc10 expression. D-F: XHoxa13 expression. G-I: XHoxd13 expression. Stage 50-52 tails were amputated at mid-tail level and left to regenerate for 1 (A,D,G) or 3 days (B,E,H). Stage 54 tails were wounded mid-tail and fixed 1 day later (C,F,I). The black arrows point to the staining in the neural tube. All pictures are side views with rostral to the left.

Gene Clone Species Stages Anatomy
hoxd13.S image:3399571 laevis NF stage 60 mesoderm , posterior , hindlimb bud , distal , blastema , [+]

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  Figure 3. Posterior Hox gene expression in regenerating limbs. Stage 54 limbs were amputated at knee level and left to regenerate for 1 (A,E,I), 3 (B,F,J), or 5 (C,G,K) days before they were subjected to in situ hybridisation. Nonregenerating stage 57 limbs were amputated at knee level and left to heal for 5 days (H,L). D: Stage 54 limb was wounded in the flank and left to heal for 1 day (black arrow points to the wound). A-D: XHoxc10 expression, (E-H) XHoxa13 expression, and (I-L) XHoxd13 expression. Hash marks indicate level of resection. Looking down dorsally, proximal to the left and anterior down.