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kcna3xenopus globe 

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Experiment details for kcna3

Expression of voltage-dependent potassium channels in the developing visual system of Xenopus laevis.

Gene Clone Species Stages Anatomy
kcna3.S laevis NF stage 37 and 38 to NF stage 40 globe

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  Fig. 2. Voltage-dependent potassium 1.3 (Kv1.3)–like subunits are expressed in the Xenopus retina. Transverse sections through the Xenopus retina at different developmental stages were immunostained with a rabbit antibody against the C-terminus of the human Kv1.3 subunit. A: At stage 32, cells in the neuroepithelium of the retina and brain are immunopositive. B: At stage 37/38, retinal ganglion cells (RGCs) and their processes along the vitreal surface are labelled, as are the inner and outer plexiform layers (IPL and OPL) and the ciliary marginal zone (CMZ). C,E: By stage 40, the optic nerve head and optic nerve are brightly labelled. D: Almost no labelling is observed when the Kv1.3 subunit antibody is preincubated with a control Kv1.3 subunit peptide antigen. Similar low power sections are shown in C and D. Br, brain; D, dorsal; L, lens; NP, neuropil; on, optic nerve; onh, optic nerve head; PE, pigmented epithelium; RGCL, retinal ganglion cell layer; V, ventral; vs, vitreal surface. Scale bar 50 m for A, B, and E and 100 m for C and D.