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kcnc4xenopus globe 

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Experiment details for kcnc4

Pollock NS et al. (2002) Assay

Expression of voltage-dependent potassium channels in the developing visual system of Xenopus laevis.

Gene Clone Species Stages Anatomy
kcnc4 tropicalis NF stage 32 to NF stage 40 globe

  Fig. 5. Voltage-dependent potassium 3.4 (Kv3.4)–like subunits are expressed in the Xenopus retina. A: Membrane-associated labelling with an anti-Kv3.4 antibody of all retinal and lens (L) cells in a stage 32 Xenopus retina. Arrow points to retinal ganglion cell (RGC) axon labelling at the vitreal surface. B,C: A stage 33/34 retina at low (B) and high (C) power shows abundant labelling of RGC processes at the vitreal surface (vs). D: In a stage 37/38 retina, membrane-associated labelling of most retinal and lens cells is maintained, and staining in the synaptic layers appears (IPL and OPL, inner and out plexiform layers). E: Low magnification micrograph demonstrates Kv3.4 immunolabelling in the brain neuropil, the optic nerve, and retina at stage 40. CMZ, ciliary marginal zone; D, dorsal; Mb, midbrain; NR, neural retina; onh, optic nerve head; V, ventral. Scale bar 50 m for A, B, and D, 100 m for E, and 20 M for C.