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Figure 3. Ion channel genes expressed in Xenopus. The inward rectifier Girk4 (kir3.4) K+ channel is expressed in neural tissue in hatched Xenopus embryos (red arrowhead) and is specifically detected in a spot (green arrowheads) on the side of the posterior head (A, close-up in B). C,D: The inward rectifier Kir6.1 is detected as a maternal message in the animal half of vegetal cells during cleavage (arrowheads). E: It is expressed in the neural tissues at somite stages but is also detected in the posterior gut (arrowheads). Maternal mRNA for the inward rectifier channel Girk1 (Kir3.1) is localized in the middle of animal cells during cleavage (arrowheads, F,G), and around the blastopore lip during gastrulation (arrowhead, H). I: Magainin mRNA is detected in the cells of the animal cap of the blastula-stage embryo (arrowheads). The K+ channel Kv1.1 can be detected by radioactive in situ hybridization in animal cells at st. 5 (arrowhead, J) and in cells undergoing ingression at the blastopore at st. 10 (K). A are chromogenic in situ hybridization, whereas J and K are radioactive sections (signal is lighter-colored, background is darker). |
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kcnj3.1 ( potassium inwardly rectifying channel subfamily J member 3) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 5 (16-cell), dorsolateral view. |
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kcnj3 ( potassium inwardly rectifying channel subfamily J member 3) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 4 (8-cell), animal view. |
