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Figure 2. Spatiotemporal expression patterns of Fin and Gill Keratin (FGK) gene during embryogenesis. A: Semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of FGK gene expression. The numbers indicate the Nieuwkoop and Faber (NF) developmental stages. Total RNA from each sample was subjected to semiquantitative RT-PCR. The ornithine decarboxylase (ODC) gene was amplified as an internal control. B: Whole-mount in situ hybridization of FGK gene expression. All photographs are lateral views. The signals of FGK expression are shown by the arrows. The arrowhead indicates the external gill at NF40. |
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krt16 (keratin 16, type I) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 28, lateral view, anterior left, dorsal up. |