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Fig. 1.
Heterochronic genes are expressed during Xenopus metamorphosis. (A) Xenopus laevis Nieuwkoop-Faber (NF) stages at pre-metamorphosis (st.50), pro-metamorphosis (st.54–57) and climax of metamorphosis (st.58–66). Bar, 1 cm. (B-G) RT-qPCR from spinal cords isolated during metamorphosis for (B) miR-125 (lin-4 orthologue), (C) let-7a, (D) lin28b, (E) trim71 (lin-41 orthologue), (F) thrβA and (G) klf9. RNA levels were normalized to 5S (B, C) and eef1α−1 (D-G) and compared to st.50 (average ±SEM, n=2–5 independent experiments). * p<0.05, ** p<0.01, t-test against hypothetical value =1 (no change compared to st.50). (H) Western blot analysis for Lin28a in brain samples. Tubulin was used as loading control. (I-N) in situ hybridization for lin28a in (I,J) spinal cord cryosections of st.50 animals using (I) sense (s) or (J) antisense (as) probes and (K-N) limbs at st.52 and st.54 using (K,L) sense (s) or (M,N) antisense (as) probes. Bar, 50 µm.
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