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Experiment details for mnx1Saha MS et al. (1997) Assay
Dorsal-ventral patterning during neural induction in Xenopus: assessment of spinal cord regionalization with xHB9, a marker for the motor neuron region.
|FIG. 4. Whole-mount in situ hybridization with xHB9. Whole mount in situ hybridization was performed on Xenopus embryos at different developmental stages. (A) Late gastrula (stage 13), lateral view, arrow depicts signal and points to the anterior-dorsal region of the embryo; the blastopore is to the right; (B) late neurula (stage 20); (C) early tailbud (stage 23); (D) late tailbud (stage 26), arrows designate retina and pituitary gland signal; dark staining in the gut cavity is background; (E) hatching stage (stage 33/34), arrow shows tailbud staining; (F) swimming tadpole (stage 37/38), arrow designates signal in retina; (G) longitudinal section through spinal cord region of a stage 35 embryo; (H) transverse section through the spinal cord region of a stage 35 embryo; (I) transverse section through the tailbud region of a stage 35 embryo; arrow designates tailbud staining. Bars in A–F correspond to 0.5 mm; bars in G–I correspond to 0.1 mm.|