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FIG. 5. Caudal expression of Noelin-1 and and HNK-1 immunostaining. 10 mm sections through embryos stained with the Z exon. Arrowheads mark neuron populations in the spinal cord that express the gene. A: Stage 28 embryo with signal in Rohon-Beard cells and interneurons. B: The same section stained with HNK-1 shows that Noelin marks only a subset of the neurons present. Some of the HNK-1 signal is quenched by the in situ reaction product. C: Stage 28 embryo with signal in interneurons and motor neurons. D: HNK-1 staining of the same section as in C. E: Stage 33 spinal cord illustrates expanding domain of Noelin-1 and -2 expression. F: Stage 35 spinal cord shows neural crest cells migrating into the fin (arrow) expressing Noelin-1 and -2. In E and F, black line demarcates the ventral neural tube. Brown cells are pigmented neural crest cells (melanocytes). no, notochord. |
