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FIG. 4. Cranial expression of Noelin-1 and -2. 10mmsections through head regions of a stage 35 embryo similar to that in Fig. 3D, stained with a probe to the Z exon. A: A midbrain-region transverse section reveals Noelin-1 and -2-positive cells in the pineal gland and brain. Arrow points to the body of the gland, arrowhead denotes cells that are not positive for Noelin-1 or -2. B: A hindbrain section at the midotic vesicle level shows signal in the VIIIth ganglion (arrow) and faintly in the IXth ganglion (arrowhead) ventral to the otic vesicle. C: Parasagittal section through the eye, Vth and VIIth ganglia and the otic vesicle. The retinal ganglion cell layer also contains signal. D: A higher-power view of a transverse section through the eye reveals transcripts in the retinal ganglion cell layer and inner nuclear layer, but not in the pigmented epithelium, photoreceptor layer, ciliary marginal zone or lens. E: A hindbrain section showing signal in the IXth ganglion. e, eye; hb, hindbrain; inl, inner nuclear layer; le, lens; mb, midbrain; rgc, retinal ganglion cell layer; V, trigeminal; VII, facial nerve and geniculate; VIII, facial-acoustic; IX, glossopharyngeal; asterisks denote otic vesicle; brown pigment is seen in melanocytes and retinal pigmented epithelium. |
