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Experiment details for penkImmunocytochemical analysis of proenkephalin-derived peptides in the amphibian hypothalamus and optic tectum.
|Fig. 2. Distribution of proenkephalin- and somatostatin-like immunoreactivity in the hypothalamus of Bufo marinus. All 4 sections are from the same toad. All 3 proenkephalin-related antisera stained perikarya, nerve fibers and terminals with the same distribution. Proenkephalin immunoreactive fibers are observed in the periventricular part of the preoptic nucleus and the lateral basal hypothalamus. The SRIF antiserum stained perikarya in the dorsal and ventral part of the preoptic nucleus. A: Met-ENK antiserum; nickel intensified. B: Met-ENK-Arg 6 antiserum; nickel intensified. C: metorphamide antiserum; nickel intensified. D: SRIF antiserum; nickel intensified. A, B, C and D ×75.|