Gene Expression Search Experiment

Search Criteria

Gene/Clone	Species	Stage	Anatomy Item	Experimenter
prc1	xenopus		chordal neural plate border [+] afferent neuron,anterior branchial crest,branchial crest,cardiac neural crest,chordal neural crest,cranial neural crest,efferent neuron,enteric neuron,hyoid crest,interneuron,mandibular crest,migratory neural crest cell,migratory trunk neural crest cell,peripheral nerve,posterior branchial crest,postmigratory neural crest cell,premigratory neural crest cell,sacral neural crest,trunk neural crest,vagal neural crest,afferent neuron,anterior branchial crest,branchial crest,efferent neuron,enteric nervous system,enteric neuron,glial cell,melanoblast,melanophore,migratory neural crest cell,neural crest,peripheral ganglion,posterior branchial crest

Too many results?Too few results?

Experiment details for prc1

Developmental regulation of central spindle assembly and cytokinesis during vertebrate embryogenesis.

Developmental regulation of central spindle assembly and cytokinesis during vertebrate embryogenesis.

Gene	Clone	Species	Stages	Anatomy
prc1.S		laevis	NF stage 13 to NF stage 15	chordal neural plate border

Display additional annotations [+]

Figure 4. PRC1 is Dynamically Expressed in the Developing Xenopus Embryo(A) In situ hybridization of PRC1 mRNA in an early-neural-plate-stage embryo. Arrowheads outline the border of the neural plate.(B) In situ hybridization of PRC1 mRNA in a late-tail-epidermis-stage embryo. Arrowheads show areas of high expression of PRC1 mRNA, including the brain, kidney, and tailbud.(C) Immunostaining of PRC1 in the tailbud epidermis. White arrow indicates the line scan indicated by the pink line in (E). Red arrowhead indicates a midbody stained by PRC1. Scale bar represents 20 μm.(D) Immunostaining of PRC1 in the neural plate. White arrow indicates the line scan (indicated by the blue line in (E). Red arrow indicates a midbody stained by PRC1. Scale bar represents 20 μm.(E) Graph of line scans for PRC1 signal intensity in representative tailbud epidermal cells (pink) and neural-plate cells (blue). Arrow and arrowhead indicate the intense signal at midbodies in both cell types.(F) Graph of the mean pixel intensity of interphase, cytoplasmic PRC1 immunstaining signal for tailbud epidermis cells (pink bar; n = 12 embryos) and neural-plate cells (blue bar; n = 7 embryos).(G) Immunostaining for PRC1 (green) and α-tubulin (red) in a tailbud epidermal cell at early telophase. PRC1 channel alone is shown in (G′); PRC1 is concentrated around bundled microtubules in the midzone. Scale bar represents 5 μm.(H) Immunostaining for PRC1 (green) and α-tubulin (red) in a neural-plate cell at early telophase. PRC1 channel alone is shown in (H′); despite cytokinesis onset, PRC is not concentrated in the midzone. Scale bar represents 5 μm.(I and J) By late telophase, PRC localizes to the central spindle in both epidermal cells (I, I′) and neural cells (J, J′).

Gene	Clone	Species	Stages	Anatomy
prc1.S		laevis	NF stage 14 to NF stage 15	chordal neural plate border

prc1 (protein regulator of cytokinesis 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 14/15, dorsal view, anterior up.