|
| Gene |
Clone |
Species |
Stages |
Anatomy |
|
rfx7.L
|
|
laevis
|
NF stage 10
|
ectoderm
,
neuroectoderm
|
|
rfx7.L
|
|
laevis
|
NF stage 14
|
ectoderm
,
neuroectoderm
,
gastrocoel roof plate
,
neural plate
|
|
rfx7.L
|
|
laevis
|
NF stage 20
|
neural plate
,
neural tube
|
|
rfx7.L
|
|
laevis
|
NF stage 25
|
brain
,
midbrain
,
hindbrain
,
somite
,
optic vesicle
,
[+]
|
|
rfx7.L
|
|
laevis
|
NF stage 29 and 30
|
brain
,
midbrain
,
hindbrain
,
spinal cord
,
optic vesicle
,
[+]
|
Display additional annotations [+]
|
| |
Fig. 1. RFX7 and RFX4 are expressed in the nervous system. (A) Spatial expression profiles of RFX7 and RFX4 genes. (B) Temporal expression profiles of RFX7 and RFX4 genes. (C) RFX7 and RFX4 are not expressed in the GRP at the stage 14. The GRP is indicated by red arrows. d: dorsal, v: ventral, a: anterior, p: posterior, b: brain, op: optic vesicles, ot: otic vesicles, sc: spinal cord, s: somites. |
|
Display additional annotations [+]
|
| |
Fig. 5. The expression of RFX4 is regulated by RFX7 and Foxj1. (A) RFX4 rescued NTD in RFX7 morphants. The bar-graphs show the quantitative assessment of the injections. At least three independent experiments were performed. “n” indicates the number of injected embryos. (B) Five hundred picograms of Foxj1 RNA was injected into a dorsal blastomere of 4-cell stage embryos and the gene expression was examined at stage 14 by whole mount in situ hybridization. β-gal staining (red) indicates the injected side. The number of embryos examined is indicated. (C) Foxj1 RNA was injected into two dorsal blastomeres of 4-cell stage embryos and the gene expression was examined at stage 14 by RT-PCR. |
