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Fig. 3. Sustained Sox2 expression promotes Müller glial cell formation. (A,B) Sox2 expression is expanded in Act β-cat transgenic embryos (A) as compared with control (B; Rx:GFP) transgenic embryos. (C-E) Lipofection of Sox2 causes a large increase in the representation of CRALBP- or R5-positive Müller cells [D, GFP, n=8, 942 cells; E, Sox2 (CRALBP), n=7, 485 cells; Sox2 (R5), n=9, 616 cells]. *P<0.001. (F-H) Sox2 causes an increase in the proportion of Müller cells born before stage 32, which are BrdU negative (G, GFP, n=5, 30 cells; H, Sox2, n=3, 31 cells). Error bars indicate s.e.m. *P<0.001. |
