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setd7xenopus neural tube [+] 

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Experiment details for setd7

The histone methyltransferase Setd7 promotes pancreatic progenitor identity.

The histone methyltransferase Setd7 promotes pancreatic progenitor identity.

Gene Clone Species Stages Anatomy
setd7.L laevis NF stage 29 and 30 neural tube

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  Fig. 1. Expression of Setd7 in the developing pancreas is conserved across vertebrate species. (A) RT-qPCR analysis of indicated transcription factors in anterior (AE) and posterior (PE) endodermal explants isolated from gastrula stage (st.) Xenopus embryos and cultured ex vivo until indicated stages. AE but not PE gives rise to pancreatic endoderm that expresses foxa2, pdx1 and ptf1a (Fig. S2) (Kelly and Melton, 2000; Horb and Slack, 2001; Spagnoli and Brivanlou, 2008). Data were normalized to that of odc and represented as fold change compared with PE sample (set to 1). n=4; error bars represent ±s.e.m. *P<0.05, **P<0.01. (B-E) In situ hybridization analysis of setd7 (B,E), pdx1 (C) and insulin (D) expression in cross-sectioned Xenopus tadpole (st. 30) embryos and dissected gut at st. 41 (E). Yellow star (*) indicates setd7 expression in the neural tube. dp, dorsal pancreatic bud; lv, liver bud; pa, pancreas. (F-J) Immunofluorescence analysis of Setd7 in the mouse endoderm and pancreas. Setd7 colocalizes with Foxa2 in the foregut endodermat E8.5 (8- to 9-somite stages) (F; dotted line demarcates the ventral foregut epithelium) and with Pdx1 in pancreatic progenitors at E10.5 (G). At E12.5 and 14.5 (H,H′,I), Setd7 is retained in the cytoplasm of pancreatic epithelium (arrowheads) and nuclear localization is detected only in delaminating cells (arrows). At E17.5 (J), Setd7 protein colocalizes with Pdx1 and insulin (Ins) in β-cell progenitors (arrows) clustering into islets. Arrowheads indicate Setd7 nuclear staining in neighbouring islet cells. Scale bars: 50 μm.