|
Display additional annotations [+]
|
|
Fig. 5. EBF2 and EBF3 are sufficient for muscle target gene expression
hGR-XEBF2 or hGR-XEBF3 mRNA were injected into one cell of two-cell stage embryos, followed by DEX treatment from the late gastrula stage (stage 11/11.5) to the neurula stage (stage 14/15). hGR mRNA was injected in control embryos. β-gal mRNA was coinjected as a marker of the injected side. In all panels the right side is the injected side, showing the blue color of X-gal staining. The (purple) expression levels of myod (B and C), m-cadherin (E and F), seb-4 (H and I), and myf5 (K and L) are strongly upregulated by EBF2 and EBF3 (brackets), while expression of hGR alone does not change the expression level of the target genes (A, D, G, and J). The expression of actin alpha (N) and tnnc1 (P) is downregulated by EBF3. All panels show dorsal views. |
|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
acta1
|
|
laevis
|
NF stage 35 and 36
to
NF stage 37 and 38
|
somite
,
heart
,
muscle
,
skeletal muscle
,
musculature of face
,
[+]
|
myf5
|
|
laevis
|
NF stage 35 and 36
to
NF stage 37 and 38
|
somite
,
musculature of face
,
hypaxial muscle
,
jaw muscle
,
periocular region
|
rbm24
|
|
laevis
|
NF stage 35 and 36
to
NF stage 37 and 38
|
lens
,
somite
,
heart
,
lateral line system
,
muscle
,
[+]
skeletal muscle
,
cranial nerve
,
lateral line placode
,
trunk lateral line
,
dorsal lateral line
,
middle lateral line
,
ventral lateral line
,
musculature of face
,
facial nerve
,
hypaxial muscle
,
jaw muscle
,
lateral line
,
muscular system
|
cdh15
|
|
laevis
|
NF stage 35 and 36
to
NF stage 37 and 38
|
somite
,
muscle
,
skeletal muscle
,
musculature of face
,
hypaxial muscle
,
[+]
|
myod1
|
|
laevis
|
NF stage 35 and 36
to
NF stage 37 and 38
|
somite
,
muscle
,
musculature of face
,
hypaxial muscle
,
epaxial muscle
,
[+]
|
|
|
Fig. 4. Expression patterns of muscle target genes
In stage 35–37 embryos myod, m-cadherin, actin alpha, seb-4, tnnc1 and myf5 are all expressed in skeletal muscle including somites (black arrows), migrating hypaxial muscle anlagen (black arrowheads) and jaw muscle (yellow arrows). myod, m-cadherin, actin alpha, seb-4, and myf5 (A-D and F) are expressed in the somites, migrating hypaxial muscle anlagen and jaw muscle, and these expression patterns overlap with those of ebf2 and ebf3 (Figure 2). m-cadherin (B) is weakly expressed in a central band in somites, with expression throughout the somite. myf5 (F) expression in somites is weaker than other genes at this stage, and is expressed at the leading edge of migrating hypaxial muscle. tnnc1 (E) is expressed in the somites. actin alpha, seb-4, and tnnc1 are expressed in the heart (yellow arrowheads). All embryos show lateral views. |
|
|
|
tnnc1 (troponin C type 1 (slow)) gene expression in Xenopus laevis embryos, NF stage 35-37, as assayed by in situ hybridization. Lateral view: anterior left, dorsal up. |