| |
Fig. 8. Darkfield photomicrographs illustrating the localization of
xTRHR3 mRNA hybridization signal in selected regions of the brain of
white-adapted Xenopus laevis. Brain sections were dipped into Kodak
NTB-2 liquid emulsion and exposed for 1 month. A: A dense accumulation
of silver grains was seen throughout the internal granular layer
of the olfactory bulb (igl). B: In the anterior preoptic area (Poa), a
moderate hybridization signal was visualized around the ventricle.
C: In the suprachiasmatic nucleus (SC), intense labeling was observed
throughout the nucleus. D: Control section, consecutive to that in C,
incubated with a sense riboprobe. E: In the magnocellular preoptic
nucleus (Mg), a moderate hybridization signal was found over a number
of cell bodies. F: In the ventral hypothalamic nucleus (VH), a
moderate density of silver grains was seen in the area lining the
ventricule. lv, Lateral ventricle. Scale bar 100 um. |
