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Gene/Clone	Species	Stage	Anatomy Item	Experimenter
ttr	xenopus

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Experiment details for ttr

Zorn AM and Mason J (2001) Assay

Gene expression in the embryonic Xenopus liver.

Gene	Clone	Species	Stages	Anatomy
ttr.L		laevis	NF stage 29 and 30 to NF stage 35 and 36	mesoderm , endoderm , alimentary system
ttr.L		laevis	NF stage 41 to NF stage 42	endoderm , alimentary system
ttr.L		laevis	NF stage 46 to NF stage 47	endoderm , liver diverticulum , alimentary system

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Fig. 2. Embryonic expression of liver markers by in situ hybridization. Each indicated probe was tested on three stages: day 7 (stage 46/47) gut tubes in the left column, day 3 (stage 41/42) gut tubes in the centre column and whole tail bud embryos (stages 30–35) in the right column. Light blue 5-bromo-4-chloro-3-indolyl-phosphate colour reactions were used to visualize the specific expression in the day 7 gut tubes. The pancreas tissue is bright white in these preparations. Brown, blue or black colour substrates indicate the specific staining in day 3 gut tubes. All preparations are shown with anterior to the left. lv, liver bud; p, pancreatic bud; gb, gall bladder bud; lu, lung bud.

Gene	Clone	Species	Stages	Anatomy
ttr.L		laevis	NF stage 33 and 34	endoderm , liver diverticulum , alimentary system

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Fig. 3. Expression of markers in the liver diverticulum endoderm. (A) Schematic of a tail bud embryo (stage 33) with the line indicating the plane of section through the liver diverticulum (left). The right side shows a typical resulting section with the endoderm indicated in grey. ot, otic vesicle; nt, neural tube; endo, endoderm; ecto, ectoderm; meso, mesoderm; lvd, liver diverticulum. (B) Representative vibritome sections (as illustrated above) of whole-mount embryos hybridized with the indicated probes. Sections from the hex, hnf3β, AMBP, endodermin, fibrinogen and transferrin stained embryos are through the anterior portion of the liver diverticulum at the level of the otic vesicle. Sox17α and transthyretin sections are from the posterior end of the liver diverticulum. All of the markers are expressed in the endodermal layer and excluded from the mesoderm. Note that the dark colour of the outer ectoderm is pigment and not staining.