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Fig. S12 Ionocyte subpopulations and marker gene expression
(A) Low dimensional visualisation of ionocyte subpopulations, overlaid with the mean expression
of Atp6v1 and carbonic anhydrase family members (same as Fig. 5D), indicating type-I (Atp6v1highCalow) and type II ionocytes (Atp6v1highCahigh). Individual expression of Atp6v1 and carbonic anhydrase family members (right panel). The scale bars indicate the scaled imputed expression of respective markers.
(B) Expression of cftr.L and cftr.S during MCE developmental stages, indicating peak expression at tailbud stages.
(C) Bulk expression from RNA-seq (TPM: transcripts per million reads) of cftr.L and cftr.S during MCE differentiation. RNA-seq captures the initial increase in expression during neurula stages and peaking at tailbud stages.
(D) Whole-mount in-situ staining for cftr.L across NF stages 12, 16, 22, 27 and 29, with zoomed- in views of stage 27 and 29. Scale bars: 1mm and 500 μm respectively.
(E) Zoomed-in view of whole-mount in-situ staining for cftr.L and foxi1.L-marked ionocytes in tailbud stage embryos. Scale bars: 100μm
(F) Distinct marking of ciliated cells (red, acetylated-tubulin (Ac-tub)) and ionocytes (black, cftr.L) in tailbud stage embryos. Scale bars: 10μm |
