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Fig. 3. Whole-mount in situ hybridization analysis of xTsg-2 and xTsg expression.
(A,C) xTsg-2 expression at stage 27 and (B,D) stage 33 embryos showing expression
in the branchial arches (ba), neural crest (nc), lateral plate mesoderm (lpm) and head
mesenchyme (hm). Embryos in C and D were made transparent in Murray�s Clearing
Solution (2:1 benzyl benzoate: benzyl alcohol) to visualize the expression of xTsg-2
localized to the midbrain-hindbrain border, the isthmus (is). D is a close up of the embryo
in B. (E) Stage 27 and (F) stage 33 embryos stained for xTsg and cleared in Murray�s
solution reveal expression in the dorsal eye (ey), heart anlage (he) and proctodeum (pro),
as well as in the posterior wall (pw) of the tailbud. cg, cement gland; fb, forebrain; hb,
hindbrain; pn, pronephros. |
