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FIG. 1. Sequence and expression of Xwnt7B. (A) Sequence alignment of Xenopus Wnt7B with mouse Wnt7 genes. (B) Spatial expression of
Xwnt7B assayed by whole-mount in situ hybridization. Gastrula embryos are viewed fromanimal (panel A) or vegetal (panel B) sides, showing
even distribution of RNA in the animal half and marginal zone at this stage. Panel C is a control embryo hybridized with a Xwnt7B sense
probe. Panel D shows Xwnt7B expression in the dorsal neural tube and the epidermal layer after neural tube closure, and panel E is a tailbud
embryo stained with a Xwnt7B sense probe. Panel F shows Xwnt7B expression in the dorsal neural tube and skin in a tailbud stage embryo.
Panel G shows expression of Xwnt7B in the dorsal midline of the neural tube and a segmented pattern of staining underneath the skin.
Panel H shows dorsal neural as well as epidermal staining in a transverse section of a tailbud embryo. Panels I and J show specific staining
of the midbrain–hindbrain boundary and the dorsal hindbrain of an embryo hybridized with Xwnt1 antisense probe. In panels D to J (except
panel H), the heads of the embryos are on the right side; panel I is a dorsal view while the others are lateral views. |