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zp2xenopus oocyte [+] 

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Experiment details for zp2

Xenopus laevis sperm-egg adhesion is regulated by modifications in the sperm receptor and the egg vitelline envelope.

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Gene Clone Species Stages Anatomy
zp2.L laevis unfertilized egg stage oocyte , egg

  FIG. 4. (A) Fluorescence micrographs of sperm binding to an oocyte, coelomic egg, dejellied oviposited egg, and fertilized egg (0.5 hr after fertilization). The sperm heads were stained with Hoechst 33342 (0.2 mg/ml) and appeared in the images as bright dots on the surface of the top, darker animal hemisphere the egg. The vegetal hemisphere appeared brighter due to autofluorescence. Because of the size of the egg (1.2–1.3 mm in diameter), not all of the sperm are in focus. Bar, 0.2 mm. (B) Fluorescence micrographs showing polyclonal anti-gp69/ 64 antibody staining of an oocyte, coelomic egg, dejellied oviposited egg, and a dejellied fertilized egg. The vegetal hemisphere appears brighter due to autofluorescence, not because of a higher level of staining by the antibody. See Materials and Methods for details.