| |
Fig. 6. Laser confocal immunolocalization of the xrds proteins.
(A) Labeling of Xenopus retina with xrds38 peptide antiserum
shows rod (r) and cone (c) outer segment immunoreactivity. For
all antibodies, rod outer segments (os) showed a striated pattern,
reflecting the labeling of disc incisures. Cone outer segments
were labeled along one side only. In this region, the stacked
cone discs form rds/peripherin-rich hairpin loops that lie in
register. (B) Labeling with xrds35 antiserum. Rod (r) but not
cone (c) outer segments show immunoreactivity. (C) Labeling
with xrds36 antiserum. Rod (r) but not cone (c) outer segments
show immunoreactivity. The myoid (m) regions of inner
segments and the synaptic terminii (s) of cones are also labeled,
but this labeling was not blockable by preincubating the
antiserum with the immunizing xrds36 peptide. Bar, 6 mm. |
